Life-stage dependent supergene haplotype frequencies and metapopulation neutral genetic patterns of Atlantic cod, Gadus morhua, from Canada’s Northern cod stock region and adjacent areas

Among extremely migratory fish species, nursery areas occupied by juveniles usually differ from grownup habitats. To higher perceive the spatial dynamics of Canada’s Northern cod stock, juveniles caught off the east coast of Newfoundland and Labrador had been in comparison with adults from the identical region in addition to people from different areas in Atlantic Canada utilizing ddRAD-derived single nucleotide polymorphisms (SNPs). A decreased proportion of homozygotes with a chromosomal inversion situated in linkage group 1 (LG1) was detected between juvenile and grownup samples within the Northern cod stock region, doubtlessly indicating age-dependent habitat use or ontogenetic choice for attributes related to the various genes situated in LG1.

No selectively neutral genetic variations had been discovered between samples from the Northern cod stock; nevertheless, important variations had been discovered between some of these samples and cod collected from St. Pierre Bank, Bay of Fundy, Browns Bank and the southern Scotian Shelf. Clustering evaluation of variants at neutral loci offered proof for 3 main genetic models: 1) the Newfoundland Atlantic Coast, 2) japanese and southern Gulf of St. Lawrence and Burgeo Bank, and 3) the Bay of Fundy, Browns Bank and southern Scotian Shelf. Both adaptive and neutral inhabitants construction inside the Northern cod stock ought to be thought-about by managers to advertise demographic rebuilding of the stock. This article is protected by copyright. All rights reserved.Alignment of the expected amino acid sequences from these genes, together with beforehand revealed subfamily members, demonstrated that these genes comprise 4 areas of the everyday MIKC-type MADS-box proteins: the MADS area, intervening (I) area and keratin-like (Okay) area, and the C-terminal area SEPⅠ and SEP Ⅱ motif.

 

[Construction of seamless genome editing system for fast-growing Vibrio natriegens]

Recently, fast-growing Vibrio natriegens, as the good potential chassis, has proven a large software in artificial biology. Genome enhancing is an indispensable instrument for genetic modification in artificial biology. However, genome enhancing instruments with excessive effectivity and constancy are nonetheless to be developed for V. natriegens artificial biology. To cope with this downside, the physiological traits of 6 V. natriegens strains had been evaluated, and CICC 10908 pressure with quick and steady progress was chosen because the host pressure for genome enhancing research. Then, the pure transformation system of V. natriegens was established and optimized.
The efficiencies of optimized pure transformation that integrates antibiotic resistance marker cat-sacB or Kan(R) onto the chromosome of V. natriegens might attain 4×10⁻⁵ and 4×10⁻⁴, respectively. Based on the optimized pure transformation, a double-selection cassette was used to realize seamless genome enhancing with excessive effectivity and constancy. The optimistic charges of 4 differing types of genetic manipulation, together with gene deletion, complementation, insertion and substitution, had been 93.8%, 100%, 95.7% and 100%, respectively. Finally, transformation and elimination of the recombinant plasmid may very well be simply achieved in V. natriegens. This work supplies a seamless genome enhancing system with excessive effectivity and constancy for V. natriegens artificial biology.
Life-stage dependent supergene haplotype frequencies and metapopulation neutral genetic patterns of Atlantic cod, Gadus morhua, from Canada's Northern cod stock region and adjacent areas
Life-stage dependent supergene haplotype frequencies and metapopulation neutral genetic patterns of Atlantic cod, Gadus morhua, from Canada’s Northern cod stock region and adjacent areas

[Construction of T7 RNA polymerase gene expression system in Anabaena sp. PCC 7120 for the expression of hG-CSF]

The low expression fee of exogenous genes in cyanobacteria is one of the bottlenecks of cyanobacteria genetic engineering. The T7 RNA polymerase expression system has achieved the environment friendly expression of exogenous genes in Escherichia coli. Cyanobacteria and E. coli are each Gram-negative micro organism with excessive genetic homology. The development of T7 RNA polymerase expression system in cyanobacteria might enhance the expression of international genes. In order to assemble the T7 RNA polymerase expression system in Anabaena sp. PCC 7120, strategies corresponding to overlapping extension PCR and digestion-ligation method had been used to assemble a site-specific integration vector pEASY-T1-F1-TacT7RNAPCmR-F2 and a shuttle expression vector pRL-T7-hG-CSF.
The site-specific integration vector is succesful of expressing T7 RNA polymerase, and the shuttle expression vector expresses hG-CSF pushed by the T7 promoter. Then we launched the site-specific integration vector into the wild kind cyanobacteria by electroporation and transferred the shuttle expression vector into the site-integrated transgenic cyanobacteria by triparental conjugative switch. In the top, we recognized the presence of international genes in cyanobacteria by PCR, examined the transcription stage of international genes in cyanobacteria by RT-PCR, and detected the protein expression of international genes in cyanobacteria by Western blotting.

4L PBS 10X

NAT1008 4L
EUR 199

10x PBS Buffer Solution

BA01802 6x100ml
EUR 97
Description: High purity buffer for various PCR applications.

10x PBS Ready Mixed Powder

BA01701 1L
EUR 68
Description: High purity buffer for various PCR applications.

10X PBS Buffer , pH 7.4

P2100-050 500ml
EUR 88

10X PBS Buffer , pH 7.4

P2100-100 1L
EUR 91

10X PBS MaxTag Histo (OKRA00043)

OKRA00043 100 ml
EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

EIA Wash Buffer 10x concentrate

1003-1 1 Liter
EUR 231

10X PBS Buffer , pH 7.4, Refill Pack

P2100-204 3.5L
EUR 138

10X PBS Buffer , pH 7.4, Refill Pack

P2100-208 8L
EUR 219

EDTA - Saline Buffer (10X Concentrate); pH 8.0

ETA010 10 L
EUR 633

EDTA - Saline Buffer (10X Concentrate); pH 8.0

ETA500 500 ml
EUR 98

EDTA - Saline Buffer (10X Concentrate); pH 8.0

ETA999 1000 ml
EUR 119

PBS 10X (WITHOUT CALCIUM AND MAGNESIUM, PH 7,4)

46-013-CM 1L/pk
EUR 90
Description: Media Catalog; Buffered Salt-Salution

SEAP (TetCMV, Puro) in PBS

LVP1184-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing puromycin selection.

SEAP (TetCMV, Bsd) in PBS

LVP1185-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Blasticidin selection.

SEAP (TetCMV, Neo) in PBS

LVP1186-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Neomycin selection.

SEAP (TetCMV, Hygro) in PBS

LVP1191-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection.

SEAP (TetCMV, Zeo) in PBS

LVP1192-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Zeomycin selection.

SEAP (EF1a, Puro) in PBS

LVP1193-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing puromycin selection.

SEAP (EF1a, Bsd) in PBS

LVP1194-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Blasticidin selection.

SEAP (EF1a, Neo) in PBS

LVP1195-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP unde EF1a promoter, containing Neomycin selection.

SEAP (EF1a, Hygro) in PBS

LVP1200-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection.

SEAP (EF1a, Zeo) in PBS

LVP1201-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Zeomycin selection.

SEAP (CAG, Puro) in PBS

LVP1202-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under CAG promoter, containing puromycin selection.

SEAP (CAG, Bsd) in PBS

LVP1203-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under CAG promoter, containing Blasticidin selection.

SEAP (CAG, Neo) in PBS

LVP1204-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP unde CAG promoter, containing Neomycin selection.

SEAP (TetCMV, GFP) in PBS

LVP1215-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with GFP fluorescent marker.

SEAP (TetCMV, RFP) in PBS

LVP1216-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with RFP fluorescent marker.

SEAP (EF1a, GFP) in PBS

LVP1217-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter with GFP fluorescent marker.

SEAP (EF1a, RFP) in PBS

LVP1218-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter with RFP fluorescent marker.

SEAP (Ubc, Puro) in PBS

LVP1219-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under Ubc promoter, containing puromycin selection.

SEAP (mPGK, Puro) in PBS

LVP1220-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under mPGK promoter, containing puromycin selection.

SEAP (ActB, Puro) in PBS

LVP1221-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under ActB promoter, containing puromycin selection.

ELISA Plate Coating buffer concentrate pH 7.4 (10X)

80050 50 ml
EUR 164

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 202

Antibody and Conjugate Diluent for ELISA concentrate (10X)

80070 50 ml
EUR 202

Stop solution for TMB substrate (ELISA) concentrate (10X)

80100 50 ml
EUR 164

SEAP (TetCMV, RFP-Bsd) in PBS

LVP1187-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with RFP fluorescent marker, containing Blasticidin selection.

SEAP (TetCMV, RFP-Puro) in PBS

LVP1188-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with RFP fluorescent marker, containing Puromycin selection.

SEAP (TetCMV, GFP-Bsd) in PBS

LVP1189-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Lentivirus express SEAP under optional inducible TetCMV promoter with GFP fluorescent marker, containing Blasticidin selection.

SEAP (TetCMV, GFP-Puro) in PBS

LVP1190-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with GFP fluorescent marker, containing Puromycin selection.

SEAP (EF1a, RFP-Bsd) in PBS

LVP1196-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter with RFP fluorescent marker, containing Blasticidin selection.

SEAP (EF1a, RFP-Puro) in PBS

LVP1197-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter with RFP fluorescent marker, containing Puromycin selection.

SEAP (EF1a, GFP-Bsd) in PBS

LVP1198-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter with GFP fluorescent marker, containing Blasticidin selection.

SEAP (EF1a, GFP-Puro) in PBS

LVP1199-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under EF1a promoter with GFP fluorescent marker, containing Puromycin selection.

SEAP (CAG, RFP-Bsd) in PBS

LVP1205-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under CAG promoter with RFP fluorescent marker, containing Blasticidin selection.

SEAP (CAG, RFP-Puro) in PBS

LVP1206-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under CAG promoter with RFP fluorescent marker, containing Puromycin selection.

SEAP (CAG, GFP-Bsd) in PBS

LVP1207-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under CAG promoter with GFP fluorescent marker, containing Blasticidin selection.

SEAP (CAG, GFP-Puro) in PBS

LVP1208-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Concentrated Lentivirus express SEAP under CAG promoter with GFP fluorescent marker, containing Puromycin selection.

CRE (Neo), CMV lentivirus, in PBS 

LVP297-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Neomycin marker. Virus concentrated and buffer changed into provided in PBS solution

lacZ (Neo) lentiviral particles in PBS

LVP333-PBS 5x107 IFU/ml x 200ul
EUR 710
Description: Concentrated lentiviral particles expressing full length lacZ enzyme (β-Galactosidase) with Neomycin marker, provided in PBS solution.

CRE (Bsd), CMV lentivirus in PBS 

LVP336-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Blasticidin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Puro), CMV lentivirus in PBS 

LVP339-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with puromycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Bsd), EF1a lentivirus in PBS 

LVP519-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Blasticidin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Puro), EF1a lentivirus in PBS 

LVP520-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with puromycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Neo), EF1a lentivirus, in PBS 

LVP521-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Neomycin marker. Virus concentrated and buffer changed into provided in PBS solution

niRFP (CMV, Puro) Lentivirus in PBS

LVP558-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus express the human codon near infred RFP under CMV promoter, containing the Puromycin resistance

niRFP (CMV, Bsd) Lentivirus in PBS

LVP559-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus express the human codon near infred RFP under CMV promoter, containing the Blasticidin resistance

niRFP (CMV, Neo) Lentivirus in PBS

LVP560-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus express the human codon near infred RFP under CMV promoter, containing the Neomycin resistance

niRFP (EF1a, Bsd) Lentivirus in PBS

LVP562-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus express the human codon near infred RFP under EF1a promoter, containing the Blasticidin resistance

niRFP (EF1a, Neo) Lentivirus in PBS

LVP563-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus express the human codon near infred RFP under EF1a promoter, containing the Neomycin resistance

CRE (Puro), CAG lentivirus in PBS

LVP573-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under CAG promoter with puromycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Bsd), CAG lentivirus in PBS

LVP574-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under CAG promoter with Blasticidin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Neo), CAG lentivirus, in PBS

LVP575-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under CAG promoter with Neomycin marker. Virus concentrated and buffer changed into provided in PBS solution

PBS Isotonic Buffer, 1 Liter; 20X Concentrate

NB303 1 Liter
EUR 276

Phosphate Buffered Saline (PBS) 10x pH 7.4, 1000 ml

12-9423-5 1000 ml
EUR 99

GFP inducible control lentiviral particles in PBS

LVP024-PBS 5x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing codon optimized GFP under tetracycline inducible suCMV promoter. Particles contain a blasticidin-RFP fusion dual marker under the constitutive RSV promoter, provided in PBS solution

CRE (GFP-Puro), CAG lentivirus in PBS

LVP576-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under CAG promoter) nuclear permeable CRE recombinase and the GFP marker under the same suCAG promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE (RFP-Bsd), CAG lentivirus in PBS

LVP577-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under CAG promoter) nuclear permeable CRE recombinase and the RFP marker under the same suCAG promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE (RFP-Puro), CAG lentivirus in PBS

LVP578-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under CAG promoter) nuclear permeable CRE recombinase and the RFP marker under the same suCAG promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

GFP (TetCMV-Puro) lentiviral particles in PBS

LVP800-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing eGFP with Puromycin antibiotic marker. Particles were concentrated and provided in PBS solution

RFP (TetCMV-Puro) lentiviral particles in PBS

LVP801-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing RFP with Puromycin antibiotic marker. Particles were concentrated and provided in PBS solution

CFP (TetCMV-Puro) lentiviral particles in PBS

LVP802-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing CFP with Puromycin antibiotic marker. Particles were concentrated and provided in PBS solution

BFP (TetCMV-Puro) lentiviral particles in PBS

LVP803-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing BFP with Puromycin antibiotic marker. Particles were concentrated and provided in PBS solution

CRE-2A-GFP, CMV lentivirus in PBS 

LVP804-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. No any antibioic selection marker. Virus concentrated and buffer changed into PBS solution.

CRE-2A-RFP, CMV lentivirus in PBS 

LVP805-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the RFP marker under the same suCMV promoter. No any antibioic selection marker. Virus concentrated and buffer changed into PBS solution.

ARE-GFP (Puro) Lentivirusin PBS

LVP981-P-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

AR-GFP (Bsd) Lentivirusin PBS

LVP912-B-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

Phosphate Buffered Saline (PBS, pH 7.4) concentrate (20X)

80081 100 ml
EUR 164

CRE-2A-RFP (Bsd), CMV lentivirus in PBS 

LVP013-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the RFP marker under the same suCMV promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-RFP (Neo), CMV lentivirus in PBS 

LVP027-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the RFP marker under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-GFP (Bsd), CMV lentivirus in PBS 

LVP337-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-RFP (Puro), CMV lentivirus in PBS 

LVP338-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the RFP marker under the same suCMV promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-GFP (Puro), CMV lentivirus in PBS 

LVP407-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-GFP (Neo), CMV lentivirus in PBS 

LVP408-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-RFP (Bsd), EF1a lentivirus in PBS 

LVP522-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-RFP (Puro), EF1a lentivirus in PBS 

LVP523-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-RFP (Neo), EF1a lentivirus in PBS 

LVP524-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Neomycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-GFP (Bsd), EF1a lentivirus in PBS 

LVP525-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suEF1a promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-GFP (Puro), EF1a lentivirus in PBS 

LVP526-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suEF1a promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

CRE-2A-GFP (Neo), EF1a lentivirus in PBS 

LVP527-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

T-Pro Washing buffer in PBS and Tween-20 (10X)

JB09-I003 500ml/BT
EUR 144

ISRE-RFP (GFP) Lentivirus in PBS

LVP938-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

ISRE-RFP (Neo) Lentivirus in PBS

LVP938-N-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ISRE-RFP (Puro) Lentivirus in PBS

LVP938-P-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ISRE-Luc (Bsd) Lentivirus in PBS

LVP939-B-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ISRE-Luc (GFP) Lentivirus in PBS

LVP939-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.
The two vectors had been efficiently constructed, the T7 RNA polymerase gene and hG-CSF gene had been transferred into cyanobacteria effectively, and each genes had been additionally expressed in cyanobacteria. In abstract, the T7 RNA polymerase expression system was efficiently constructed in cyanobacteria, and the expression fee of hG-CSF gene was doubled than the standard cyanobacteria expression techniques. This expression system will present a greater instrument for the applying of cyanobacteria genetic engineering and will promote the event of cyanobacteria as a chassis cell within the fields of artificial biology sooner or later.