Cusabio C-terminal Avi-tagged Recombinant

R&D Systems offers a wide variety of biotinylated proteins that allow researchers to take the guesswork out of protein biotinylation. In addition to amine-tagged biotinylated proteins, we have a growing list of C-terminal Avi-tagged Recombinant biotinylated proteins. Each biotinylated protein is held to the same industry-leading quality standards as its non-labelled counterpart while limiting variability from batch to batch. In addition, R&D Systems’ custom protein development team can work with you to create a custom protein solution that meets your specific research needs.

Advantages of Biotinylated Avi-tag Recombinant proteins

  • Consistent labelling. Biotinylation only occurs at the single lysine residue in the Avi tag.
  • Uniform protein orientation. When bound to a streptavidin-coated surface, the orientation of the tagged protein will be uniform due to precise control over biotinylation.
  • Equivalent bioactivity. Because biotinylation is restricted to the Avi tag, the rest of the protein is not changed, so there is no interference with protein bioactivity.

Recombinant Human 5T4 His-tag Avi-tag Protein, CF AVI10290-100: R&D Systems


Recombinant human HSP90α (heat shock protein 90) spanning amino acids 535-732 (end). This construct contains an N-terminal His tag (6xHis) followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using Avi-Tag™ and affinity purified.

Construct: HSP90α (His-Avi-535-732 (end) – (biotin)

Species: Human

Host species: E. coli

MW: 25kDa

Genbank: #NM_005348

Tag(s): N-terminal His-tag, N-terminal Avi-tag ™


This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation was confirmed to be ≥90%.

Amino acids: 535-732(end)

UniProt: #P07900

Applications: Useful for the study of enzyme kinetics, detection inhibitors and selectivity profiles.

Active Biotinylated Proteins Avi-Tag - Protein Assay Formats: R&D Systems

Storage stability

At least 6 months at -80°C


Heat shock protein 90 alpha, HSP90a, HSP90alpha, HSP90 alpha, HSP90AA1, heat shock protein 90 alpha family class A member 1, EL52, HSPN, lipopolysaccharide-associated protein 2, LPS-associated protein 2, LAP2, LAP-2, heat shock 86 kDa, HSP86, HSPC1, HSPCA

Purity: ≥90%

Format: Aqueous buffer solution

Test conditions

The assay was performed according to the HSP90α C-Terminal Domain TR-FRET Assay Kit (BPS Bioscience #50289) with HSP90α titrated from 48 ng/rxn to 0 ng/rxn.


40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol