R&D Systems offers a wide variety of biotinylated proteins that allow researchers to take the guesswork out of protein biotinylation. In addition to amine-tagged biotinylated proteins, we have a growing list of C-terminal Avi-tagged Recombinant biotinylated proteins. Each biotinylated protein is held to the same industry-leading quality standards as its non-labelled counterpart while limiting variability from batch to batch. In addition, R&D Systems’ custom protein development team can work with you to create a custom protein solution that meets your specific research needs.
Advantages of Biotinylated Avi-tag Recombinant proteins
- Consistent labelling. Biotinylation only occurs at the single lysine residue in the Avi tag.
- Uniform protein orientation. When bound to a streptavidin-coated surface, the orientation of the tagged protein will be uniform due to precise control over biotinylation.
- Equivalent bioactivity. Because biotinylation is restricted to the Avi tag, the rest of the protein is not changed, so there is no interference with protein bioactivity.
Recombinant human HSP90α (heat shock protein 90) spanning amino acids 535-732 (end). This construct contains an N-terminal His tag (6xHis) followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using Avi-Tag™ and affinity purified.
Construct: HSP90α (His-Avi-535-732 (end) – (biotin)
Host species: E. coli
Tag(s): N-terminal His-tag, N-terminal Avi-tag ™
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation was confirmed to be ≥90%.
Amino acids: 535-732(end)
Applications: Useful for the study of enzyme kinetics, detection inhibitors and selectivity profiles.
At least 6 months at -80°C
Heat shock protein 90 alpha, HSP90a, HSP90alpha, HSP90 alpha, HSP90AA1, heat shock protein 90 alpha family class A member 1, EL52, HSPN, lipopolysaccharide-associated protein 2, LPS-associated protein 2, LAP2, LAP-2, heat shock 86 kDa, HSP86, HSPC1, HSPCA
Format: Aqueous buffer solution
The assay was performed according to the HSP90α C-Terminal Domain TR-FRET Assay Kit (BPS Bioscience #50289) with HSP90α titrated from 48 ng/rxn to 0 ng/rxn.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol